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Image Search Results
Journal: Frontiers in Cell and Developmental Biology
Article Title: Down-Regulation of Lnc-CYP7A1-1 Rejuvenates Aged Human Mesenchymal Stem Cells to Improve Their Efficacy for Heart Repair Through SYNE1
doi: 10.3389/fcell.2020.600304
Figure Lengend Snippet: Expression profile of lncRNAs in young and old hBM-MSCs. (A) lncRNA expression in old (O) and young (Y) hBM-MSCs was determined by microarray analysis. (B) Differential lncRNA expression was validated by real-time qPCR. n = 5/group; ∗ P < 0.05 O vs. Y.
Article Snippet: Total RNA was isolated from hBM-MSCs after cultured in hypoxic conditions for 72 h. 12 ×
Techniques: Expressing, Microarray
Journal: Frontiers in Cell and Developmental Biology
Article Title: Down-Regulation of Lnc-CYP7A1-1 Rejuvenates Aged Human Mesenchymal Stem Cells to Improve Their Efficacy for Heart Repair Through SYNE1
doi: 10.3389/fcell.2020.600304
Figure Lengend Snippet: The expression of SYNE1 was inhibited by lnc-CYP7A1-1. (A) Gene co-expression networks were built to detect the interactions among lncRNAs and genes. Red color indicates expression up-regulated and green color indicates expression down-regulated. The dots represents genes and the diamond represents lncRNAs. (B) The expression of SYNE1 in old (O) and young (Y) hBM-MSCs was determined by microarray analysis. (C) The expression of SYNE1 in Old (O) and young (Y) hBM-MSCs was compared by real-time qPCR. (D) Old (O) hBM-MSCs were transfected by inhibition lentivirus (O-sh-CYP7A1) or control lentivirus (O-c), respectively. The expression of SYNE1 was compared by real-time qPCR. n = 5/group; * P < 0.05 O vs. Y, O-c vs. O-sh-CYP7A1.
Article Snippet: Total RNA was isolated from hBM-MSCs after cultured in hypoxic conditions for 72 h. 12 ×
Techniques: Expressing, Microarray, Transfection, Inhibition, Control